ActiveSight is a company focusing on early lead discovery using fragment-based drug design and world-class X-ray crystallography expertise and equipment. ActiveSight is a division of Rigaku Americas Corporation which gives its employees excellent stability, pay and benefits and is based in San Diego, California.

Open Positions

Research Associate, Protein Biology

Description

The candidate will join our fast paced research group in molecular biology and protein purification. The position entails producing and expressing recombinant proteins to support crystallographic efforts for structure based small molecule drug design.

Requirements

The ideal candidate will have the following background/skill sets:

• Bachelors, or Masters, in molecular biology, biochemistry, or related discipline.
• Must be highly organized, motivated and able to work independently in a collaborative team-oriented setting
• Excellent analytical communication and organizational skills are a must.
• Minimum of 2 years experience in the following areas:
• Cloning including PCR, restriction analysis, DNA sequencing, primer and vector design of clones for use in E. coli and/or baculovirus expression systems. Experienceith Baculovirus cell cultures (including Wave bioreactor fermentations) and transfection techniques a plus
• Protein expression analysis, optimization, and scale-up (6-15L)
• Protein purification of recombinant proteins including but not limited to: His-tag, GST-tag, Maltose affinity purification, Ion Exchange, Hydrophobic Interaction, Size Exclusion
• Protein refolding
• Purification of untagged proteins
• Western blotting and SDS-PAGE techniques
• Assay Development: Fluorescence, TRF, Luminescence, PF, Couple assay

To Apply

To apply, send your resume to jobs@active-sight.com